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Objective:To evaluate the efficacy and safety of injectable compound solution of hyaluronic acid combined with botulinum toxin in the treatment of perioral wrinkles.Methods:A total of 60 female patients, aged 45-60 (53±5) years, with perioral wrinkles treated by the above mentioned method in Plastic Surgery Hospital, CAMS, PUMC from January 2018 to December 2020 were reviewed. Injectable compound solution of hyaluronic acid (2.5 ml) and botulinum toxin were injected intradermally into the upper and lower lips. Injectable compound solutions of hyaluronic acid (1.5 ml and Hearty Xiongmaozhen) were injected intradermally in the perioral static wrinkles and the lip border, respectively, as needed. The patients were followed up for 3 to 6 months after treatment. Photographs were taken before the treatment and during the follow-up visits. The pre- and post-treatment severity of perioral wrinkles were evaluated by two independent surgeons. Patients′satisfaction was evaluated.Results:All the 60 patients were included. The severity of perioral wrinkles was significantly reduced after the treatments. All the patients were satisfied with the results. Among them, 45 patients (75%) were very satisfied and 15 patients (25%) were satisfied with the results. Mild swelling occurred in all patients and was relieved in 1-5 days. Mild bruising was observed in 9 patients (15%) and was relieved in 7-10 days. No other complications were documented.Conclusions:Injectable compound solution of hyaluronic acid combined with botulinum toxin is a safe and effective method in the treatment of perioral wrinkles.
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Objective:To observe the clinical effect, postoperative satisfaction and complications of liposuction combined with low-energy bipolar radiofrequency BodyTite in arm with dermatolysis.Methods:From June 2013 to December 2019, 66 female patients were included. Firstly, liposuction treatment was performed in the designed operation area, and then radio-frequency skin tightening treatment was performed with BodyTite equipment. All patients were followed up for 6 months.Results:All the 66 patients had completed the operation successfully; their age ranged from 22-53 (28.3±6.9) years. The average body mass index was (23.8±3.4) kg/m 2, the average liposuction volume of unilateral upper arm was (288.6±95.6) ml, the average radiofrequency energy of unilateral upper arm was (4.2±1.1) kJ and the average operation time was (75.1±18.7) min 6 months after operation. A total of 42 cases were followed up. By self-evaluation satisfactory rate of patients was 92.8%, and the satisfactory rate of third-party independent plastic surgeons was 88.1%. There were no hematoma, seroma or infection except one case of skin blister. There was no serious complication requiring further surgical intervention. Conclusions:Bipolar radiofrequency BodyTite is a safe and effective method for the treatment of fat accumulation of upper arm with flabby skin.
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Objective@#To investigate the clinical efficacy of circumferential thigh liposuction with three incisions in thigh.@*Methods@#From April 2017 to October 2018, 31 patients received circumferential thigh liposuction with three incisions. All patients were females at 17-42 years old, with the mean age of 25.6± 0.8 years. An incision was designed in the pubic area, two in right and left inferior gluteal folds respectively. After injecting tumescent liquid into operation area, blunt and bent cannulas with 2.5-3.5 mm in diameter and 20-45 cm in length were used to extract deep subcutaneous fat, but 0.5 cm thick subcutaneous superficial fat was preserved. Elastic pants were dressed for 3 months after surgery. The thigh contour improvement was evaluated by the patients and 2 doctors.@*Results@#All the patients recovered uneventfully, without complications of fat embolism or infection. Satisfaction ratios of overall thigh and inner thigh contour increased from 81.7% to 80.6%, evaluated by patients. Ten patients completed postoperative outpatient revisit after 2 months. Satisfaction ratios of overall thigh and inner thigh contour increased from 80% to 75%, evaluated by doctors.@*Conclusion@#Three-incision circumferential thigh liposuction is an effective method in thigh contouring with concealed incision scar.
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Objective@#Report the experience of autologous fat grafting forfacial contouring and rejuvenation patients.@*Methods@#Retrospective analysis the treatment of 450 facial contouring and rejuvenation patients with global optimization autologous fat grafting in Plastic Surgery Hospital of Chinese Academy of Medical Sciences from January 2011 to December 2017. Using low negative pressure liposuction technical and blunt cannula (diameter 2.5 mm and 2.0 mm), different fat particle was harvested and concentrated by a cotton pad. The one-hole blunt cannula was used for fat grafting with multi tunnel and multi-point and two layer(deep and superficial)injection ways, regularly following up and taking picture for imaging evaluation.@*Results@#All patients were successfully performed with fat grafting. Grafted fat volume was 15-65 ml. There were 132 cases of local skin bruising after operation, which gradually subsided about 2 weeks, no severe complications occurred. 450 patients were followed up for 6-12 months. Facial contour and skin texture were improved without subcutaneous nodules and irregularity. The outcome of all patients were satisfactory. Of a total of 450 patients, 319 (70.89%) were evaluated as very good result, 94 (20.89%) as good result, and 37(8.22%) asregular result.@*Conclusions@#Optimization autologous fat grafting can improve the facial contouring and rejuvenation with little complications and satisfactory results.
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Objective To report our experience of breast augmentation patients with fat grafting.Methods The treatment of 560 patients with fat grafting for breast augmentation was summarized in our hospital from Jan.2011 to Dec.2015.Fat was harvested using low negative pressure liposuction technique with 3-hole blunt cannula (diameter 2.5 mm).The fat was managed using cotton pad for concentration of fat tissue and separating them from fluids,oil,and debris.A diameter 2.5 mm,1-hole blunt cannula was used to place the fat through 3-mm incision on inframammary fold,fat grafting with multi-level and multi-tunnel and multi-point injection ways,regularly taking picture for imaging evaluation.Results All patients were successfully performed with breast augmentation,and no severe complications occurred.Grafted fat volume ranged from 180 to 400 ml (average,235 ml) per breast.Most of women had a significant improvement in their breast size and shape postoperatively,and the breasts were soft and natural in appearance.The patients were followed up for 18-24 months,and the outcome were satisfactory.Conclusions Autologous fat grafting for breast augmentation simplifies the operation procedure with satisfied results (natural breast and body contouring) and avoids the complications of breast prosthetic procedures.
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Objective@#To observe the outcome of breast reconstruction with autologous fat grafting in the patients following treatment for breast cancer.@*Methods@#The clinical data of 22 patients after breast cancer modified radical mastectomy with fat grafting for breast reconstruction from January 2012 to March 2015 at Department of Body Contouring and Liposuction Center of Plastic Surgery, Hospital of Peking Union Medical College were analyzed retrospectively. The age of 22 patients (all female) was 28 to 54 years. Fifteen patients were performed breast modified radical mastectomy 5 to 16 year ago without radiotherapy, 7 patients were performed breast modified radical mastectomy following regular radiotherapy 2 years ago. Low negative pressure liposuction technical was applied to harvest fat tissue for 400 to 800 ml which was filtrated and purified by cotton pad method in low temperature environment. Fat grafting was performed with multi-level and multi-tunnel and in multi-point injection ways. All patients were followed up by regular imaging evaluation with MRI or ultrasonography after operation every 3 months.@*Results@#All breast reconstruction were successfully performed in 22 patients, no severe complications occurred. Among 15 patients without radiotherapy, 12 patients were performed with autologous fat grafting for breast reconstruction, 3 patients with prosthetic implantation for breast augmentation after autologous fat grafting. Among 7 patients with radiotherapy, 6 patients were performed with autologous fat grafting for breast reconstruction, 1 patient with prosthetic implantation for breast augmentation after autologous fat grafting. The volume of fat grafting was 104 to 380 ml. It took 2.5 hours to finish the operation including 1.0 to 1.5 hours for liposuction and 40 minutes for fat grafting. Next fat grafting were performed after 3 months. The fat of the breast were survived well detecting by MRI, only 1 patient had a cystic nodule which had been resected during nipple reconstruction. Ultrasonography screened several cystic nodules with the major axis of 0.1 to 0.2 cm in the breast, which couldn′t be found by palpating in 18 patients. The patients were followed up for 18 to 36 months, the outcome were satisfactory.@*Conclusion@#Autologous fat grafting for breast reconstruction simplifies the operation program with satisfied results and avoids the complications of breast reconstruction with skin flap.
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Objective To explore the feasibility and validity of the new photograph method for blepharoplasty,which was designed for collecting information of patients originally,such as eyelid abnormalities and poor appearance.Methods A lot of 1068 cases were collected and taken pictures preand post-operation,including six postures of looking straight forward,upward,downward,closing,squeezing and smiling,and then compared with photos taking in traditional way,only looking forward and closing.Results Compared with the traditional method,it contained more details of eyelids by six-position photography in front view,which was beneficial to the comparison of eyelid skin,muscle stretch,adhesion and dynamic changes before and after the operation.Conclusions More static and dynamic information about eyelid can be collected by simple six-position photography in front view.It is appropriate for photograph collection of blepharoplasty,with important clinical significance.
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<p><b>OBJECTIVE</b>To investigate the emergency treatment for large amputated ear defect.</p><p><b>METHODS</b>From Feb. 2010 to Oct. 2013, 5 cases with large unilateral amputated ear defects were treated. The amputated auricular cartilage was replanted subcutaneously in mastoid area at the first stage. Cranioauricular sulcus was reconstructed and skin grafting was performed at the second stage.</p><p><b>RESULTS</b>All cases were followed up for 3-6 months with satisfactory result. The reconstructed helix, scapha and cavity of auricular concha had similar color and elasticity as the ear at healthy side. Scar at donor sites, such as inguinal region and armpit, was inconspicuous.</p><p><b>CONCLUSIONS</b>The method of auricular cartilage replantation is an effective way for emergency treatment of large amputated auricular defect with less morbidity at donor site.</p>
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Adolescente , Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Cartilagem da Orelha , Transplante , Orelha Externa , Ferimentos e Lesões , Cirurgia Geral , Seguimentos , Reimplante , Resultado do TratamentoRESUMO
Objective To study the expression of gonadotropin releasing hormone receptor (GnRH receptor) in the stomach of Sprague-Dawley rat exposed to light stress. Methods We established illumination stressed models that the rats were exposed to continuous light(totally 64 rats, 32 rats for experiment and control group, respectively). Then,stomachs were taken from the rats when the rats exposed to continuous light for 1 day, 2 days, 3 days, 4 days, 1 week, 2 weeks, 3 weeks, 4 weeks and their control groups, respectively.The localization and the expression of GnRHR and GnRHR mRNA in stomachs mucous membrane were detected using immunohistochemistry, Western blotting and real-time PCR. Results Immunoreactivity was displayed mostly in the parietal cells in gastric gland. There were not differences of this distribution in all groups of constant illumination and their control. The immunoreactive materials were distributed on membrane and in cytoplasm of all positive cells, but not in nuclei. The level of GnRHR in rats exposed to continuous illumination were higher from one week to four weeks compared with that in control(P<0.05), and the level of GnRHR reached the peak when rats were exposed to constant illumination for two weeks (P<0.01). The quantity of GnRH mRNA in rats exposed to continuous illumination were higher from three weeks to four weeks compared with that in control(P<0.05). Conclusion The expression of GnRHR in digestive tract was effected by illumination stress.GnRH might regulate digestive function by interaction with GnRHR, suggesting that GnRH may be acted as a hormone, not only responded to normal physiological function of digestive tract but also responded to stress activity.
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Eight-academic-year medical students will have the degree of Medical Doctor-when they graduate. Teachers should combine the theoretical knowledge and scientific research thinking in the teaching of physiology,consequently enlightening the students'research conscious-ness.The article aims to introduce this teaching mode from the example of coronary circulation,hoping to be helpful to the perfection of eitht-year medical program education.
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@#ObjectiveTo investigate the in vitro effect of ghrelin on membrane membrane potentail of GH3 cells.MethodsNystatin-perforated whole-cell recording configuration was used.ResultsThe membrane potentail of GH3 was decreased from-(72±4.78) mV to(54.8±4.58) mV(P<0.01) after application of ghrelin(10-7～10-8 M)ConclusionGhrelin can transiently and significantly decreased the amplitude of membrane potentail,which may contribute to the ghrelin-stimulated GH secretion from GH3 cells.
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@#ObjectiveTo identify the influence of cholesterol on insulin-secreting cells.Methods MIN6 mouse insulin cells were cultured in vitro. After confluence of cells, on one hand, free cholesterol in series concentrations was added to cultu re medium to act for 24 hours, and on the other hand, free cholesterol in same c oncentration was added to culture medium to act for different periods. MTT test was used to evaluate the viability of MIN6 cells.Results25μmol/L free cholesterol significantly decreased the viability of MIN6 c ells after 24 hours and 100μmol/L free cholesterol significantly decreased the viability of MIN6 cells after 12 hours and induced cells death after 24 hours.Conclusions Free cholesterol decreases viability of MIN6 cells in a dose-dependent and time-dependent manner, and it is indicated that elevated free cholesterol concentration in blood may be one factor involved in the dysfunction of pancreatic β-cells.
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0.05);the epididymal indexes were significantly increased 6,24 and 72 hours after formalin injection(P
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<p><b>OBJECTIVES</b>To investigate the method of preparing porcine thoracic aortas acellular tissue matrix (ACTM) by trypsin, EDTA and Triton X-100 and to find the best concentration of X-100.</p><p><b>METHODS</b>A total of 56 roots of fresh thoracic aortas (without adventitial tissue) from 80 kg-100 kg tame pigs were divided randomly into > groups, each containing 8 roots. Every vessel was put into a 50 ml centrifugal tube with a solution of 0.1% trypsin + 0.02EDTA in PBS for 24 h. After that, each group was separately immerged into a solution of 0.1%, 0.2%, 0.5%, 1.0%, 2.0%, 5.0%, 10.0% Triton X-100 for 144 h-240 h. Specimens were taken every 6 h. Specimens were stained with haematoxylin-eosin and observed grossly under the light and transmission electron microscopy.</p><p><b>RESULTS</b>Light and transmission electron microscopy revealed that ACTM was composed of insoluble collagen, elastin, and some insoluble metamorphic organelles. The best concentration of Triton X-100 was 1% at the time of 176.25 h +/- 5.5 h.</p><p><b>CONCLUSIONS</b>Porcine thoracic aortas ACTM can be obtained successfully through this procedure. Triton X-100 is a good reagent for preparing vessel ACTM.</p>
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Animais , Aorta Torácica , Biologia Celular , Cirurgia Geral , Prótese Vascular , Octoxinol , Farmacologia , Suínos , Engenharia Tecidual , MétodosRESUMO
Objective To discuss the problems of wire-LAN-based HIS and the implementation of wireless LAN.Methods Several technologies,such as security technology,anti-jamming technology,were used to implement wireless LAN.Results Wireless LAN,without the disadvantages of wire LAN,played a more and more important role in HIS.Conclusion Wireless LAN,being mature from the aspect of technology,is being popularized.